Circadian oscillations of RPCH gene expression in the eyestalk of the crayfish Cherax quadricarinatus.

نویسندگان

  • Francisco Martínez-Pérez
  • Samuel Zinker
  • Guadalupe Aguilar
  • Jesús Valdés
  • Hugo Aréchiga
چکیده

The RPCH and beta-actin cDNAs from the crayfish Cherax quadricarinatus were amplified, cloned and sequenced. The primary structure sequences of these cDNAs were compared to other members of the AKH/RPCH family. Fluctuations in the amount of the C. quadricarinatus RPCH and beta-actin mRNAs, as cDNAs, were quantified every 3h by RT-PCR. Single cosinor analysis supports the notion of beta-actin and RPCH mRNA circadian behavior in animals subjected to 12h:12h light/dark regimes. In constant darkness RPCH mRNA concentration changes to ultradian cycles.

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عنوان ژورنال:
  • Peptides

دوره 26 12  شماره 

صفحات  -

تاریخ انتشار 2005